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. Author manuscript; available in PMC: 2017 Jul 18.
Published in final edited form as: Chem Res Toxicol. 2016 Jun 15;29(7):1098–1107. doi: 10.1021/acs.chemrestox.5b00528

Figure 6.

Figure 6

Antioxidants attenuate reactivity of GAPDH with DOPAL. % Control refers to the integrated staining density of the GAPDH monomer relative to untreated sample. All incubation times were 4 h versions (0.3 mg/mL GAPDH, 50 µM DOPAL, and 5 mM antioxidant in 50 mM sodium phosphate buffer, pH 7.4, 37 °C). GAPDH alone was significantly different from GAPDH with DOPAL (n = 13) as determined by one way ANOVA with Neuman Keuls multiple comparison post test (* = p < 0.001). GAPDH with antioxidants were all also significantly different from GAPDH with DOPAL. GAPDH and DOPAL with GSH (n = 7) or with AscH− (n = 6) were significantly different from GAPDH with DOPAL. GAPDH and DOPAL with Trolox or with NaCNBH3 showed no significant difference from GAPDH and DOPAL.