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. 2017 Jan 10;59(1):2–14. doi: 10.1111/jipb.12504

Figure 3.

Figure 3

GmSIZ1a and GmSIZ1b rescue the Arabidopsis siz1‐2 mutant phenotype (A) and (B) Morphological phenotype of wild‐type, siz1‐2, and complementation plants. GmSIZ1a (#5‐1, #8‐2 and #10‐1) and GmSIZ1b (#3‐7, #10‐3 and #15‐1) indicate lines expressing GmSIZ1a:GFP or GmSIZ1b:GFP driven by the Arabidopsis SIZ1 promoter in the siz1‐2 background, respectively. Vector indicates empty vector transformed into siz1‐2 plants. Bars = 1 cm. (C) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) analysis of Arabidopsis PR1, PR2, PR5, and ICS1 expression in Col‐0, siz1‐2, and complementation lines under normal conditions. Relative expression was normalized to that of AtUBC and data represent the mean ± SD, n = 3. (D) Seed germination rate of Col‐0, siz1‐2, GmSIZ1a #5‐1, and GmSIZ1b #10‐3 in the presence of the indicated concentrations of abscisic acid (ABA) at 4 days after planting. Data are the mean ± SD, n = 3. (E) Percentage of green cotyledons in Col‐0, siz1‐2, GmSIZ1a #5‐1, and GmSIZ1b #10‐3 seedlings at 7 days after planting in the presence of ABA. Data are the mean ± SD. Three biological replicates were performed.