Figure 1.

Characterization of strains used in this study. (A) Sequence alignment of human and Saccharomyces cerevisiae Naa10. Protein sequences were aligned using PROMALS3D(Pei et al., 2008) and the crystal structure of Naa10 from Saccharomyces pombe (PDB structure 4KVX)(Liszczak et al., 2013) was used for secondary structure prediction. The alignment matrix between human and yeast Naa10 reached a 49.5% identity score. The homologous position to hNaa10 S37P (yeast Serine 39) is marked with an arrow. (B) RT‐PCR characterization of the human replacement strains using primers designed to amplify regions internal to the indicated transcripts. The products of these reactions are shown, in the presence and absence of reverse transcriptase. (C) Western blot analyses with antibodies for the human and yeast Naa10 and Naa15 along with yGAPDH as a loading control for the strains where the yNaa10 has been modified, (D) for the humanized strains, expressing the human proteins from the endogenous locus and (E) for the strains overexpressing the human proteins from plasmids