Table 1.

Yeast strains used in this work

Strain		Background	MAT	Genotype	Source	Number
Endogenous strains	WT	W303‐1A	A	leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15	RS	YG2
	ΔyNaa10	W303‐1A	A	ard1△::KanR leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15	RS	YG1
	ΔyNaa10::URA3‐15	W303‐1A	A	ard1△::URA3 leu2‐3,112 trp1,1 ura3‐1 ade2‐1 his3‐11,15	RS	YG10
	△yNaa15	W303‐1A	alpha	nat1‐5::LEU2 leu2‐3,112 trp1 ura3‐1 ade2‐1 his3‐11,15	RS	YG3
	ΔyNaaa10/ΔyNaa15	W303‐1A	A	ard1△::URA3 nat1△::LEU2 leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15	this work	YG67
	yNaa10 S39P	W303‐1A	A	leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15 ard1‐S39P	this work	YG36
	yNatA::hNatA WT	W303‐1A	A	leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15 nat1△::hNaa15 ard1△::hNaa10	this work	YG42
	yNatA::hNatA S37P	W303‐1A	A	leu2‐3,112 trp1‐1 can1‐100 ura3‐1 ade2‐1 his3‐11,15 nat1△::hNaa15 ard1△::hNaa10‐S37P	this work	YG43
hNatA overexpression	WT [e. v.]	W303‐1A and B‐8961	A	ade2‐1 ura3‐1 his3‐11,15 leu2‐3,112 trp1‐1 can1‐100 p[BEVY URA3]	TA	YG33
	ΔyNatA [e. v.]	W303‐1A and B‐8961	A	ade2‐1 ura3‐1 his3‐11,15 leu2‐3,112 trp1‐1 can1‐100 ard1△::LEU2 nat1△::KanMX p[BEVY URA3]	TA	YG35
	ΔyNatA [hNatA WT]	W303‐1A and B‐8961	A	ade2‐1 ura3‐1 his3‐11,15 leu2‐3,112 trp1‐1 can1‐100 ard1△::LEU2 nat1△::KanMX p[BEVY hNAT1 hARD1 URA3]	TA	YG32
	ΔyNatA [hNatA S37P]	W303‐1A and B‐8961	A	ade2‐1 ura3‐1 his3‐11,15 leu2‐3,112 trp1‐1 can1‐100 ard1△::LEU2 nat1△::KanMX p[BEVY hNAT1 hARD1‐S37P URA3nn	TA	YG34
tester	Mating tester	—	A	lys1	AN	YG62
	Mating tester	—	alpha	lys1	AN	YG63

Abbreviations: RS, Rolf Sternglanz; TA, Thomas Arnesen; AN, Aaron Neiman.