TABLE 2.
Kd values for AgrA binding to the P2 and P3 promoter constructs of the RNAIII-agr intergenic regiona
Parameter | Values (nM) for AgrA binding to:
|
|||
---|---|---|---|---|
P2 without acetyl phosphate | P2 with acetyl phosphate | P3 without acetyl phosphate | P3 with acetyl phosphate | |
Kd1 | 0.23 ± 0.04 | NA | 0.75 ± 0.05 | NA |
Kd2 | 3.8 ± 1.6 | 0.16 ± 0.03 | 2.2 ± 0.8 | 1.7 ± 0.3 |
Kds are listed in order of increasing size of the protein/DNA complex. Data shown are averages of two determinations. NA, not applicable.