FIG. 2.

Expression and purification of Rv2361c. An expression vector was constructed in which Rv2361c is fused to a histidine repeat at the N terminus and used to transform E. coli BL21(DE3)pLysS. Expression was induced by the addition of 1 mM IPTG, and the protein was purified by IMAC chromatography. Fractions eluted from the column were subjected to SDS-PAGE, and proteins were visualized by staining with Coomassie brilliant blue 250R. Lane 1, uninduced soluble cell extract. Lane 2, soluble cell extract after IPTG induction. Lane 3, purified Rv2361c.