TABLE 1.

Plasmids and bacterial strains used in this study

Plasmid or strain	Relevant characteristic(s)	Source or reference
Plasmids
p1523	HP1523 fragment (bp 343-1520) in pGEMT-Easy	This work
pVacA	VacA fragment (bp 798-2003) in pGEMT-Easy	This work
pRecA	RecA fragment (bp 1-1040) in pGEMT-Easy	This work
pRecGKm	HP1523::aphA in pGEMT-Easy	This work
pVacAKm	vacA::aphA in pGEMT-Easy	This work
pRecAKm	recA::aphA in pGEMT-Easy	This work
pVacA-0	vacA with 0-bp deletion cassette	6
pVacA-50	vacA with 50-bp deletion cassette	6
pVacA-100	vacA with 100-bp deletion cassette	6
pRecG-0	HP1523 with 0-bp deletion cassette	This work
pRecG-50	HP1523 with 50-bp deletion cassette	This work
pRecG-100	HP1523 with 100-bp deletion cassette	This work
pAD1	ureA promoter	4
pAD1-HPrG	pAD1 with HP1523	This work
pAD1-ECrG	pAD1 with E. coli recG	This work
Strains
E. coli
AB1157	recG+ (wild type)	11
N4452	AB1157 recG::cat	29
N4452-AD1	N4452 with pAD1 (control)	This work
N4452-HPrG	N4452 complemented with HP1523	This work
N4452-ECrG	N4452 complemented with E. coli recG	This work
H. pylori
JP26	Wild-type strain
JP26/HP1523::aphA	ΔHP1523	This work
JP26/vacA::aphA	ΔVacA	This work
JP26/recA::aphA	ΔRecA	This work
JP26 HPrG	HP1523::aphA complemented with HP1523	This work
JP26 ECrG	HP1523::aphA complemented with E. coli recG	This work
JP26/HP1523:0	HP1523 interrupted with 0-bp deletion cassette	This work
JP26/HP1523:50	HP1523 interrupted with 50-bp deletion cassette	This work
JP26/HP1523:100	HP1523 interrupted with 100-bp deletion cassette	This work
JP26/vacA::0	vacA interrupted with 0-bp deletion cassette	6
JP26/vacA::50	vacA interrupted with 50-bp deletion cassette	6
JP26/vacA::100	vacA interrupted with 100-bp deletion cassette	6