TABLE 2.
Primers used in this study
| Primer | Gene | Locationa | Sequence (5′ → 3′)b |
|---|---|---|---|
| F343 | HP1523 | 1553772-1553794 | ATCCTTACCGAGTTTGGCAAGA |
| R1520 | HP1523 | 1552617-1552637 | GAAATGCCCACCTCAATGAG |
| R483 | HP0153 (recA) | 163411-163430 | TTGGATCCGCATGTGGGCTTGCAAGCA |
| F477 | HP0153 (recA) | 163385-163405 | CTTGGATCCCCATCAATCTCCGCTTTAGG |
| F1 | HP0153 (recA) | 162928-163405 | ATGGCAATAGATGAAGACAAAC |
| R1040 | HP0153 (recA) | 163944-163968 | TCCATTTCTTCTAAAGGCTCATCC |
| R1060 | HP0887 (vacA) | 939456-939475 | TACGGGATCCTTTTCCTGCTTGAATGCGC |
| F1082 | HP0887 (vacA) | 939497-939517 | AAGAGGATCCAAGGAATACGACTTATACA |
| F798 | HP0887 (vacA) | 939213-939231 | CGTGAAAGCGAAAAACAAG |
| R2003 | HP0887 (vacA) | 940400-940418 | CATCGGCTTTAGTGTTGA |
a
HP1523 locations based on the sequence of strain J99. HP0153 (recA) and HP0887 (vacA) locations based on the sequence of strain 26695.
b
BamHI restriction sites underlined.