TABLE 2.

Kinetic properties of A. fulgidus AlaDH

Assay and substrate	kcat (s−1)	Km (mM)	kcat/Km (M−1 s−1, 103)	Ki (mM)
Oxidative deaminationa
l-Alanine	6.1	0.71	9	16.8
l-2-Aminobutyrate	9.6	0.085	113	4.7
NAD+	6.1	0.60	10	NIb
Reductive aminationa
Pyruvate	118	0.16	739	27.8
Ketobutyrate	143	0.48	298	4.8
Oxaloacetate	113	0.97	116	NI
NADHd	118	0.02	5,900	0.12
NH4+	118	17.3	7	NDc

^aThe assay was performed at pH 8.5 (titrated at 25°C) and 82°C.

^bNI, no inhibition.

^cND, not determined.

^dWith pyruvate as the substrate.