TABLE 3.

Substrate specificity for the oxidative deamination reaction of AlaDH

Substratea	Sp actc (U/mg)
l-Alanine	10.6 ± 0.6
l-2-Aminobutyrateb	16.4 ± 1.0
l-Valine	1.30 ± 0.05
l-Serine	1.06 ± 0.05
l-Threonine	0.85 ± 0.05
l-Isoleucine	0.42 ± 0.02
l-Aspartate	0.42 ± 0.02
l-Lysine	<0.01
l-Tyrosine	<0.01
l-Proline	<0.01
l-Tryptophan	<0.01
l-Phenylalanine	<0.01

^aSubstrate concentration, 5 mM.

^bl-2-Aminobutyrate concentration, 1 mM.

^cThe assays were performed at pH 8.5 (titrated at 25°C) and 82°C.