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Substrate specificity of the reductive amination reaction of AlaDH
| Substrate (concn, mM) | Sp acta (U/mg) |
|---|---|
| Pyruvate (5) | 203 ± 12 |
| 3-Fluoropyruvate (5) | 40.6 ± 2.4 |
| Hydroxypyruvate (5) | 20.3 ± 1.2 |
| Oxaloacetate (1) | 195 ± 12 |
| Ketobutyrate (1) | 246 ± 15 |
| Ketovalerate (10) | 12.2 ± 0.7 |
| Ketocaproate (10) | 4.1 ± 0.2 |
| Phenylpyruvate (10) | <0.1 |
The assays were performed at pH 8.5 (titrated at 25°C) and 82°C.
