Table 1.

Nras^G12D/+p53^−/− MEPs are transformed to leukemia-initiating cells

Donor cell types	No. of donor cells	No. of helper cells	No. of recipient mice	Disease diagnosis	No. of diseased animals	%
WBM	2.5 × 105	2.5 × 105	20	AML and T-ALL	20	100
CD45+ BM	2.5 × 105	2.5 × 105	4	AML and T-ALL	4	100
HSC	30	2 × 105	10	AML and T-ALL	7	70
MPP	30	2 × 105	12	AML and T-ALL	10	83
MP	10 000	2 × 105	15	AML	8	53
GMP	4000	2 × 105	8	N/A	0	0
MEP	2000	2 × 105	14	AML	10	71

Primary Nras^G12D/+p53^−/− mice were injected with pI-pC as described in “Materials and methods.” Various types of cells were isolated on day 12 and transplanted with helper cells (CD45.1⁺) into lethally irradiated mice. Mice without any diseases were monitored for at least 200 days.

BM, bone marrow; WBM, whole bone marrow.