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Expression of exsA::lacZ (A) and exoS::lacZ (B) in strains PAK, PAKmucA22, PAKalgU::Gm, and PAK harboring algU overexpression plasmid pWW025. Bacteria were grown in LB (white bars) or LB plus 5 mM EGTA (black bars) to an OD600 of 1 to 2 before β-galactosidase assays. *, P < 0.05.
