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. 2004 Nov;186(22):7575–7585. doi: 10.1128/JB.186.22.7575-7585.2004

FIG. 4.

FIG. 4.

Expression of exsA::lacZ (A) and exoS::lacZ (B) in the backgrounds of PAK, PAKmucA22, PAKalgR::Gm, and PAKalgR::Gm complemented with algR-expressing plasmid pWW022. For algR gene complementation, various concentrations of IPTG were added into the culture medium as indicated. Bacteria were grown in LB (white bars) or LB plus 5 mM EGTA (black bars) to an OD600 of 1 to 2 before β-galactosidase assays. *, P < 0.05.