TABLE 5.

Genes down regulated in PAKmucA22

ID no.a	Gene	Function	Fold change in mucA22 vs wild type	TSBb (fold)	LBb (fold)
PA3450		Adaptation, protection	−3.5	1.84	1.17
PA2138		DNA replication, recombination, modification, and repair	−3.2	−2.50	−3.19
PA0523	norC	Energy metabolism	−3.0	−1.70	−1.7
#PA3445		Hypothetical	−3.9	2.66	1.59
#PA3446		Hypothetical	−5.1	1.36	1.57
PA3931		Hypothetical	−3.8	1.91	1.22
PA0281	cysW	Membrane proteins, transport of small molecules	−3.5	1.18	1.13
PA0282	cysT	Membrane proteins, transport of small molecules	−3.0	−1.10	−1.07
PA1601		Putative enzymes	−3.5	1.35	−1.44
PA3444		Putative enzymes	−5.1	1.11	−1.51
PA1246	aprD	Secreted factors (toxins, enzymes, alginate); protein secretion-export apparatus	−3.4	2.05	−2.64
PA1312		Transcriptional regulators	−3.1	−1.10	−1.55
PA3927		Transcriptional regulators	−5.1	−1.70	−1.67
PA0198	exbB1	Transport of small molecules	−5.5	2.71	−3.68
PA0280	cysA	Transport of small molecules	−6.0	−1.00	−1.18
PA2204		Transport of small molecules	−4.7	1.16	−1.16

^a#, up regulated in mucA mutant compared to PAK but down regulated in PAK under type III-inducing conditions versus noninducing conditions and vice versa. Not included are those known to be affected by the growth medium, such as those varied in TSB versus LB (44).

^bChange in gene expression in PAK grown under T3SS-inducing conditions versus PAK grown under non-T3SS-inducing conditions (44). Bacteria were grown in TSB or LB.