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. 2016 Dec;55(6):889–898. doi: 10.1165/rcmb.2015-0237OC

Figure 2.

Figure 2.

Silencing HIF-1α in hypoxic SAECs recovers CD55. (A) HIF-1α is silenced in 6-hour hypoxic SAECs transfected with 50 nM HIF-1α small interfering RNA (siRNA) versus 6-hour hypoxic SAECs with control siRNA (50 nM) or in normoxia. Lamin β1 was the loading control. (B) HIF-1α was repressed in 24-hour hypoxic SAECs transfected with HIF-1α siRNA versus control siRNA or normoxic levels. Data represent means (±SEM); n = 3; *P < 0.05 versus normoxia or control siRNA in 24 hours of hypoxia; one-way ANOVA with Bonferroni posttest. (C) CA9 transcripts were repressed in HIF-1α siRNA–transfected 6-hour hypoxic SAECs versus 6-hour hypoxic SAECs with or without control siRNA. Values represent means (±SEM); n = 3; **P < 0.01 versus nomoxia, 6 hours of hypoxia, or control siRNA; one-way ANOVA with Bonferroni posttest. (D) CD55 transcripts were recovered in HIF-1α siRNA–transfected 6-hour hypoxic SAECs versus 6-hour hypoxic SAECs in the presence or absence of control siRNA. Values represent means (±SEM); n = 3; *P < 0.05 versus nomoxia, 6 hours of hypoxia, or control siRNA; one-way ANOVA with Bonferroni posttest. (E) CD55 was assessed in normoxic, 24-hour hypoxic HIF-1α siRNA, or control siRNA–transfected SAECs. Vinculin was the loading control. (F) CD55 was recovered in 24-hour HIF-1α siRNA SAECs versus control siRNA or normoxic levels. Values represent means (±SEM); n = 3; *P < 0.05 versus normoxia, 6 hours of hypoxia, or control siRNA; one-way ANOVA with Bonferroni posttest.