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. 2016 Dec;55(6):767–778. doi: 10.1165/rcmb.2016-0006OC

Figure 2.

Figure 2.

Lung matrix metalloproteinase 9 (MMP-9), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), elastin, and keratinocyte growth factor 7 expression after intraperitoneal LPS injection. Lungs were harvested from NOX2+/+ and NOX2−/− mouse pups injected with intraperitoneal LPS or saline after 24 or 96 hours for protein analysis. (A) Homogenized lung lysates were used to immunoblot MMP-9 and TIMP-1 at 24 hours; β-actin was used as a loading control. (B) MMP-9 quantification by densitometry is shown graphically. *P < 0.01 (NOX2+/+ control versus NOX2+/+ i.p. LPS); #P < 0.01 (NOX2+/+ i.p. LPS versus NOX2−/− i.p. LPS) (n ≥ 4). (C and D) Elastin (C) and fibroblast growth factor 7 (FGF7) (D) mRNA expression was quantified by real-time polymerase chain reaction 96 hours after intraperitoneal LPS injection. *P < 0.05 (NOX2+/+ control versus NOX2+/+ i.p. LPS); #P < 0.02 (NOX2+/+ i.p. LPS versus NOX2−/− i.p. LPS) (n ≥ 4). (E and F) Clarified lung lysates obtained 96 hours after LPS treatment in pups were blotted for FGF7 and elastin (E), with quantification by densitometry shown graphically (F). *P < 0.01 (NOX2+/+ control versus NOX2+/+ i.p. LPS); #P = 0.01 (NOX2+/+ i.p. LPS versus NOX2−/− i.p. LPS) (n ≥ 4). ANOVA with post hoc tests was used for analysis of data.