Figure 4.
P. acnes drives MyD88-dependent proinflammatory mediator production. Wt and Myd88−/− mice were administered 109 viable P. acnes intratracheally and were killed after (A) 3 days or (B) 6 hours to look at early cellular recruitment and proinflammatory mediator production. Single-cell suspension of lung digest was stained for myeloid cell types: AM, TM/MO, DC, and PMN. Six hours after P. acnes administration, the lungs were homogenized and analyzed for the level of CXCL1, CXCL2, and IL-1α in lung homogenate. (B) Background levels of naive lung homogenate were subtracted from each mouse genotype. Statistical significance was determined using multiple t tests (A) and two-way analysis of variance with Sidak’s multiple comparisons (B). ***P < 0.0005, ****P < 0.0001. CXCL, CXC chemokine ligand.