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Time of feeding, number of ticks and tick gender from which salivary glands were removed into RNAlater for library construction.
| Fed | Females | Males |
|---|---|---|
| 0 | 4 | 4 |
| 60–90 min | 4 | 4 |
| 230–260 min | 4 | 4 |
| 6–6.5 hr | 4 | 4 |
| 15 hr | 4 | 4 |
| 1 day | 4 | 4 |
| 2 days | 3 | 3 |
| 3 days | 3 | 3 |
| 4 days | 3 | 3 |
| 5 days | 3 | 3 |
| 6 days | 3 | 3 |
| 7 days | 3 | 3 |
| 8 days | 3 | 3 |
| 9 days - females dropped off | 3 | 3 |
| Total | 48 | 48 |
