TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| W3110 | Wild type | ATCC 27325 |
| BW545 | Δ(lacU)169, rpsL | Lab. collection |
| AH218 | Δ(focA-pflB)-FRT-Km-FRT | 37 |
| AH243 | W3110 Δ(focA-pflB)-FRT, Km | This study |
| AH245 | Δ(araFGH)-Km | W3110 × P1(BW27269) |
| AH247 | W3110 Δ(araE)-Km | This study |
| AH248 | Δ(focA-pflB) Δ(araFGH)-Km | AH243 × P1(BW27269) |
| AH249 | Δ(focA-pflB) ΦPCP13-araE+ | AH243 × P1 (BW27752) |
| AH250 | Δ(focA-pflB) Δ(araE)-Km | AH243 × P1(AH247) |
| AH257 | W3110 Δ(xylE)-FRT-Km-FRT | This study |
| AH280 | W3110 Δ(xylG)-FRT-Km-FRT | This study |
| BW18499 | Δ(codB-lacI)3 zfb-223::Tn10 ackA202 rpoS396(Am)? rph-1 ΔglnL2001 | CGSC; 10 |
| BW25113 | lacIqrrnBT14 ΔlacZW116 hsdR514 araBADAH33ΔrhaBADLD78 | B. Wanner |
| BW27269 | BW25113 Δ(araFGH)-Km903 | CGSC |
| BW27752 | BW25113 Φ(ΔaraEp PCP13-araE) | CGSC; 16 |
| BW25141(pKD4) | p(FRT-Km-FRT) | B. Wanner |
| BW25113(pKD46) | BW25113 p(λ-γβexo under ParaB)-repA101ts | B. Wanner |
| DH5α(pCP20) | p(FLPase) | B. Wanner |
| LCB898 | pflB1 | CGSC; 35 |
| SE1265 | LCB898, zca::Tn10 | This study |
| SE1900 | BW545, pflB-1 zca::Tn10 | BW545 × P1(SE1265) |
| SE2358 | W3110 Δ(focA-pflB)-FRT-Km-FRT | W3110 × P1(AH218) |
| YK19 | ackA202 zfb-223::Tn10 | W3110 × P1(BW18499) |
| E. coli B | Wild type | ATCC 11303 |
| SE2273 | E. coli B, pflB1 | This study |
| Plasmids | ||
| pAH286 | pBR322-(PCP6-xylE+) Apr | This study |
| pAH291 | pTOPO-(xylE+) Apr | This study |