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Zn2+, proinsulin, and insulin staining of hZnT8 RW male mice (line T-12) after an HFD from 6 to 16 weeks of age. Pancreatic sections (10 μm) from three different 16-week-old HFD-fed hZnT8 T-12 Tg+ mice (top panels) and three different HFD-fed Tg− mice (bottom panels) were stained with anti-proinsulin (exposure time 100 ms), anti-insulin (exposure time 500 ms), and ZP1 (exposure time 200 ms) at magnification ×100. Scale bar represents 400 μm.
