Table 1.

Quantitation of threshold-integrated density of proinsulin, insulin, and Zn²⁺ staining in RW and WT Tg+ and Tg− mice fed an HFD or ND

	ZP1	Insulin	Proinsulin
RW HFD Tg+	458 ± 83a1,c1,d1	479 ± 52.6a2,c2,d2	199 ± 20a3,c3
RW HFD Tg−	782 ± 87	343 ± 30.6	284 ± 78
RW ND Tg+	804 ± 120b1	121.4 ± 18b2	116 ± 8.9b3
RW ND Tg−	620 ± 116	50.9 ± 9.6	240 ± 35
WT HFD Tg+	828 ± 138e1,f1	48.1 ± 9.6e2,f2	204 ± 21.3e3
WT HFD Tg−	466 ± 57	80.5 ± 9.2	137 ± 16.4
WT ND Tg+	301 ± 56	31.2 ± 6.4	205 ± 8.5
WT ND Tg−	646 ± 78	31.2 ± 11	206 ± 33

Values are the average × 10⁴ ± SEM in relative fluorescence units of threshold-integrated density from 3–4 independent experiments (n = 15–25).

^a1,a2,a3 indicate a significant difference between ZnT8RW Tg+ and Tg− mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

^b1,b2,b3 indicate a significant difference between ZnT8RW and ZnT8WT Tg+ mice fed a ND in pancreatic ZP1, insulin, and proinsulin staining, respectively.

^c1,c2,c3 indicate a significant difference between ZnT8RW Tg+ mice fed an HFD vs. ND in pancreatic ZP1, insulin, and proinsulin staining, respectively.

^d1,d2 indicate a significant difference between ZnT8RW and ZnT8WT Tg+ mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

^e1,e2,e3 indicate a significant difference between ZnT8WT Tg+ and Tg− mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

^f1,f2 indicate a significant difference between ZnT8WT Tg+ and Tg− mice fed a ND in pancreatic ZP1 and insulin staining, respectively.