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. 2017 Jan 20;12(1):e0170374. doi: 10.1371/journal.pone.0170374

Fig 3. Sensitivity of the mRT-PCR assay.

Fig 3

(A) Lanes 1–7: Individual equine-origin H3N8 (A/canine/Colorado/6723-8/2008), hH3N2 (A/Jiangxi/262/2005), H1N1/2009 (A/canine/Beijing/cau2/2009), and cH3N2 (A/canine/Beijing/364/2009) viruses were tested at 10-fold dilutions ranging from 1 × 105 to 1× 10−1 TCID50/100 μl. Lane 8: negative control allantoic fluid. (B) Lanes 1–6: A mix of the same four viruses were tested using serial 10-fold dilutions from 1 × 105 to 1 × 100 TCID50/100 μl. Lane M: molecular marker; Lane 7: negative control allantoic fluid.