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. 2017 Jan 20;12(1):e0170335. doi: 10.1371/journal.pone.0170335

Fig 1. Design of the experimental infections of mallards with different subtypes of LPAIV.

Fig 1

Fifteen 1-month-old mallards were inoculated with the H3N8 LPAIV via choanal cleft and randomly divided into three challenge groups with five ducks each. Group 1 was sequentially challenged with H4N6, H10N7 and H14N5 LPAIV at 1-month interval each. Group 2 was challenged with H10N7 two months after H3N8 inoculation, while group 3 was challenged with H14N5 after three months. Single infection and age-matched control ducks were included for all inoculations (Fig 1).