Fig 3. Western blot analysis shows a reduced amount of cellulase (CBHI) at both neutral and alkaline pH.

The analysis was performed using supernatants collected from the N. crassa 74A, Δmus-52, and Δpac-3 strains after growth for eight days on sugarcane bagasse at pH 3.0, 5.0, 7.0, 8.0, and 10.0. Anti-cellulase antibody from Trichoderma viride were used to detect CBH-1.