Table 1. qRT-PCR primers used in this study.
GENES | Sequence 5′→3′ (Forward) | Sequence 5′→3′ (Reverse) |
---|---|---|
cbh-1 | ACCCCCTCCACCAACG | GTGAACGCTGTAGAGACTTTGT |
gh7-1 | GCCCTACTCCAGCATTCAT | GTGAAGGCGGTGGCG |
gh3-2 | GGTGGAGAGGATGGATGAT | CCATCTGTTCGTTCGTCC |
gh11-1 | GGCTCTTACTCTGTGAACTGG | GTAGCCGTTGCCCGAG |
gh10-4 | GGGTGGGAGGAATGAAGA | ATCCTCGCCGCACACA |
gh43-5 | GAATGTTTATCAGGGCACG | GCTCATACCCAGTCCCG |
act | ATGTCGACGTCAGGAAGGATCT | GAGCAGTGATCTCCTTCTGCAT |
clr-1 | GTTGTATTGTGCTTCTCGTCTTG | AACGCTGTTGGAGGTCATC |
clr-2 | ACGCAGTGTGGTTTCTCTAC | TCCAATACCGCCTCAATCTG |
cre-1 | GAGGATCACGATGACCACTATG | GAGTAGCAAGTGGAGTGTGATC |
xlr-1 | AGAGTACCACAGCAACCAAG | TTCGCTCATATCGCTCATGG |
cbh-1 = Cellobiohydrolase 1 (exoglucanase) NCU07340; gh7-1 = Endoglucanase 1 NCU05057; gh3-2 = β-glucosidase 1 NCU08054; gh11-1 = Endo-1,4-β-xylanase 1 NCU02855; gh10-4 = Endo-1,4-β-xylanase 2 NCU07130; gh43-5 = β-xylosidase NCU09652. clr-1 = Cellulose degradation regulator-1 NCU07705; clr-2 = Cellulose degradation regulator-2 NCU08042; cre-1 = Carbon catabolite regulation NCU08807; xlr-1 = Xylan degradation regulator-1 NCU06971; act = actin NCU04173