TABLE 1.
Constructs used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| BL21(DE3)pLysS | For overproduction of proteins | Novagen |
| NovaBlue | Recipient for cloning experiments | Novagen |
| Salmonella strains | ||
| JR501 | For converting E. coli plasmids to Salmonella compatibility | 33 |
| SJW1103 | Wild type for motility | 41 |
| SJW1364 | flhA mutant (Δ917-929, frameshift) | 15 |
| Plasmids | ||
| pET19b | N-terminal His tag T7 expression vector | Novagen |
| pGEX-2T | GST fusion expression vector | Amersham |
| pTrc99A | Expression vector | Amersham |
| pTrc99A-FF4 | Modified pTrc expression vector | 30 |
| pFFF1300 | pET19b/His-FliF | 7 |
| pGFBN269A | pET19b/His-FlhBN269A | 8 |
| pGS20 | pET19b/His-FliO | G. Schoenhals, unpublished |
| pJM200. . . pJM218 | pTrc99A-FF4/FlhAΔ1 (residues 328-357). . .pTrc99A-FF4/FlhA Δ19 (residues 688-692) | This study |
| pJM219 | pTrc99A-FF4/FlhAΔFHIPEP | This study |
| pJM272 | pTrc99A/His-FlhAΔ18QWQIL22 | This study |
| pJM273 | pTrc99A/His-FlhAΔ1-22 | This study |
| pJMA1 | pET19b/His-FlhA | This study |
| pJMP1 | pGEX-2T/GST-FliP (residues 22-245)-His | This study |
| pJMQ1 | pGEX-2T/GST-FliQ-His | This study |
| pJMR1 | pGEX-2T/GST-FliR-His | This study |
| pMM101 | pTrc99A-FF4/His-FLAG-FlhAc | 27 |
| pMM108 | pTrc99A/His-FLAG-FlhA | T. Minamino, unpublished |
| pMM130 | pTrc99A-FF4/FlhA | 14 |
| pMM309 | pTrc99A-FF4/FliH | 26 |
| pMM310 | pET19b/His-FliH | 27 |
| pMM404 | pTrc99A-FF4/FliJ | 21 |
| pMM405 | pET19b/His-FliJ | 27 |
| pMM1701 | pET19b/His-FliI | 27 |
| pMM1719 | pTrc99A-FF4/FliI | 26 |