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. 2017 Jan 20;12(1):e0169932. doi: 10.1371/journal.pone.0169932

Fig 7. Exosomes isolated from plasma of high grade glioma patients inhibit CD3+T cells proliferation through an effect mediated by CD14+monocyte.

Fig 7

(A) PBMCs were stimulated with anti-CD3 and anti-CD28 in the absence (white column, CTRL) or presence (black column, GBM-EXO) of different dilutions of exosomes isolated from plasma of high grade glioma patients (GBM). Healthy donor plasma-derived exosomes were used as control (striped column, EXO-HEALTHY). (B) PBMCs or CD14 negatively-sorted PBMCs (CD14-) were stimulated with anti-CD3 and anti-CD28 in the absence (white column, CTRL) or presence (black column, GBM-EXO) of exosomes isolated from plasma of GBM, 1:10 dilution. Proliferation of CD3+ was measured by CFSE assay on day 4. Columns, mean (n = 6); bars, SD;*, significantly different from the control; P<0.05.