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. 2017 Jan 20;12(1):e0170093. doi: 10.1371/journal.pone.0170093

Fig 4. Effect of FS on the intensity of pERK expression in PFCx: peroxidase immunolabelling.

Fig 4

(A) Representative micrographs showing pERK immunostaining in pyramidal neurons of the upper layers of the ILCx of RLA animals under basal condition and after FS. Arrowheads indicate pERK-expressing neurons with different level of intensity: black arrowhead at higher intensity and white arrowhead at lower intensity. (B, C) Quantitative analysis of pERK-labeling intensity in the PrLCx (B) and the ILCx (C). PrLCx: RHA Bs = 0.11 ± 0.02; RLA Bs = 0.10 ± 0.02; RHA FS = 0.16 ± 0.01; RLA FS = 0.12 ± 0.01; two-way ANOVA: (line) F(1,18) = 1.50, n.s.; (FS): F(1,18) = 3.74, p = 0.06; (line x FS interaction) F(1,18) = 1.45, n.s.); ILCx: RHA Bs = 0.13 ± 0.02; RLA Bs = 0.13 ± 0.02; RHA FS = 0.19 ± 0.02; RLA FS = 0,16 ± 0,02; two-way ANOVA: (line) F(1,18) = 0.51, n.s.; (FS): F(1,18) = 5.65, p = 0.03*; (line x FS interaction) F(1,18) = 0.32, n.s. Number of animals in each experimental group: RHA Bs, n = 6; RLA Bs, n = 6; RHA FS, n = 5; RLA FS, n = 5. Scale bar = 20 μm.