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. 2017 Jan 20;12(1):e0170093. doi: 10.1371/journal.pone.0170093

Fig 6. FS increases the phosphorylation of histone H3 in the PrLCx and ILCx of RLA and RHA rat lines.

Fig 6

(A and B) Representative micrographs showing pH3 immunohistochemical labeling in the PrLCx (A) and the ILCx (B) of each experimental group. (C, D) Quantitative analysis of pH3 labeled cell density in PrLCx (C) and ILCx (D). PrLCx: RHA Bs = 109.8 ± 28.1; RLA Bs = 127.5 ± 13.4; RHA FS = 288.8 ± 44.6; RLA FS = 338.6 ± 30.7; two-way ANOVA: (line) F(1,18) = 1.17, n.s.; (FS) F(1,18) = 39.01, p < 0.0001***; (line x FS interaction) F(1,18) = 0.26, n.s.; ILCx: RHA Bs = 97.4 ± 23.7; RLA Bs = 100.8 ± 16.6; RHA FS = 202.1 ± 18.9; RLA FS = 214.0 ± 9.9; two-way ANOVA: (line) F(1,18) = 0.16, n.s.; (FS) F(1,18) = 32.76, p < 0.0001***; (line x FS interaction) F(1,18) = 0.05, n.s. Number of animals in each experimental group: RHA Bs, n = 6; RLA Bs, n = 6; RHA FS, n = 6; RLA FS, n = 5. Scale bar = 50 μm.