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. 2017 Jan 20;12(1):e0170093. doi: 10.1371/journal.pone.0170093

Fig 7. Differential effects of FS on the intensity of pH3 immunostaining in the PrLCx and the ILCx.

Fig 7

(A) Representative high magnification images showing pH3 immunostaining in ILCx of RLA animals under basal conditions and after FS. Arrowheads indicate pH3-expressing neurons with different immunolabeling intensity (black arrowhead: higher intensity; white arrowhead: lower intensity). (B, C) Quantitative analysis of pH3-labeling intensity in PrLCx (B) and ILCx (C). PrLCx: RHA Bs = 0.06 ± 0.004; RLA Bs = 0.06 ± 0.004; RHA FS = 0.12 ± 0.005; RLA FS = 0.10 ± 0.009; two-way ANOVA: (line) F(1,18) = 4.93, p = 0.03$; (FS) F(1,18) = 70.41, p < 0.0001***; (line x FS interaction) F(1,18) = 3.48, p = 0.07; ILCx: RHA Bs = 0.07 ± 0.003; RLA Bs = 0.06 ± 0.003; RHA FS = 0.13 ± 0.003; RLA FS = 0.11 ± 0.002; two-way ANOVA: (line) F(1,18) = 11.85, p < 0.003$ $; (FS): F(1,18) = 154.81, p < 0.0001***; (line x FS interaction) F(1,18) = 1.26, n.s. Bonferroni post-hoc test FS p < 0.05§. Student’s t-test for independent samples: p < 0.05◊). (D-G) Quantitative analysis of pH3-labeling intensity distributions in the PrLCx (D, E) and the ILCx (F, G) of each experimental group. PrLCx χ2 test: RHA Bs > RLA Bs, p < 0.05#; RHA FS > RLA FS, p < 0.001###; ILCx χ2 test: RHA Bs > RLA Bs, p < 0.001###; RHA FS > RLA FS, p < 0.01##). Number of animals in each experimental group: RHA Bs, n = 6; RLA Bs, n = 6; RHA FS, n = 5; RLA FS, n = 5. Scale bar = 20 μm.