TABLE 1.
Effect of the igaA1 mutation on the expression of PhoP-PhoQ-activated (pag) genes
| Transcriptional fusion | β-Galactosidase activity (Miller units)a
|
||
|---|---|---|---|
| Wild type | igaA1 | phoP | |
| pbgD::MudJ | 157.0 | 63.8 | 6.2 |
| mgtC::MudJ | 49.1 | 24.8 | 3.0 |
| psiD::MudJ | 59.5 | 43.1 | 7.9 |
| pbgO::MudJ | 144.7 | 67.7 | 14.4 |
| pcgF::MudJ | 375.8 | 166.8 | 35.0 |
| pcgG::MudJ | 203.7 | 81.6 | 5.0 |
| pcgL::MudJ | 157.7 | 93.7 | 25.7 |
| pbgX::MudJ | 152.0 | 77.5 | 17.3 |
| zzz::MudJR9b | 490.6 | 448.3 | 576 |
a
The data are data from a representative experiment from a total of three independent assays.
b
The zzz::MudJR9 transcriptional fusion was used as an internal control. This transposon was inserted into a noncharacterized locus and was selected at random from colonies showing a strong blue color in 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) LB indicator plates.