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. 2017 Jan 20;12(1):e0170106. doi: 10.1371/journal.pone.0170106

Fig 3. Vps13 mutant flies show a decreased life span, age dependent impairment of locomotor function and neurodegeneration.

Fig 3

(A) Life span analysis of isogenic control and Vps13 mutant flies. (B) The fraction of dead flies of total flies used,observed within the indicated time intervals. (C) Life span curve of Vps13 mutant flies and three excision lines. (D) Climbing behavior was analyzed by determining the percentage of isogenic control and Vps13 mutant flies (4 and 17 days old) able to climb 5 cm against gravity within 15 seconds. Mean and SEM are plotted (n = 5). For statistical analysis a two-tailed students T-test was used. P<0.001 is ***. (E) Fly heads (20 day old) of control and homozygous Vps13 mutant flies were fixed, dehydrated and embedded in epon. Sections, visualizing a cross section of the complete brain, were stained with toluidine blue. The scale bar indicates 50 μm.(F) Higher magnification images of the boxed area’s in Fig E. The central complex is denoted with a dotted line. The scale bar indicates 25 μm.