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. 2017 Jan 20;12(1):e0170504. doi: 10.1371/journal.pone.0170504

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© 2017 Martin et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Evaluation of the mAb 2E4E4 specificity against swine and bovine CD40 was performed by immunocytometric analysis: A. Analysis of mAb 2E4E4 and a defined IgG1 control mAb by PAGE; B. HEK-293A cells transfected with a construct expressing full length swine CD40 and probed with 2E4E4; C. HEK-293A cells transfected with a construct expressing full length bovine CD40 and probed with the mAb 2E4E4; and D. Sham treated HEK-293A cells probed with the mAb 2E4E4. Flow cytometric analysis performed on: E. HEK-293A cells transfected with a construct encoding full length swine CD40; or F. full length bovine CD40 probed with either the mAb 2E4E4 (Red) or IgG1 isotype control (Blue).