Table 1. MHC-II upregulation by swine PBMCs stimulated with the mAb 2E4E4.
Sample | MHCII % positive cells |
---|---|
LPS | 19.0 |
0 μg/mL (2E4E4) | 8.77 |
0.5 μg/mL (2E4E4) | 11.9 |
1 μg/mL (2E4E4) | 14.9 |
2.5 μg/mL (2E4E4) | 16.6 |
5 μg/mL (2E4E4) | 22.2 |
Swine PBMCs were incubated with the mAb 2E4E4 or LPS for 24hr. and MHC-II upregulation was determined by flow cytometric analysis. Data is presented as absolute percentage of MHCII positive cells compared to IgG1 isotype control.