Skip to main content
. 2017 Jan 20;12(1):e0170054. doi: 10.1371/journal.pone.0170054

Fig 1. Modulation of Ust expression in melanoma cell lines.

Fig 1

(A) Total RNA and cell lysates of B16V control, mock transfected B16V (B16Vmock) and clones of B16VshUst were analyzed by qRT-PCR. Ust expression was normalized to the housekeeping genes β-actin and ubiquitin. (B) Immunoblots of protein lysates were probed for Ust and β-actin of different transfected B16V melanoma cell lines (lower panel). Band intensities were quantified and Ust signals were normalized to β-actin (upper panel). (C) Sulfotransferase activity of the cell lines B16V, B16VshUst(6) and B16VshUst(16). (D) Highly-sulfated cell surface CS/DS disaccharide composition of the cell lines B16V, B16VshUst(6) and B16VshUst(16). Data shown are the mean±SEM (n≥4); **, P<0.01, ***, P<0.001).