Table 1. Expression of integrins in B16 cell lines.

Gene	Protein	Mock	B16VshUst(6)	B16VshUst(16)
Itgb1	β1 integrin	1.43 ± 0.37	2.28 ± 0.32	3.6 ± 0.5
Itgb3	β3 integrin	1.37 ± 0.25	2.23 ± 0.18	2.64 ± 0.37

B16V, B16V^mock, B16V^shUst(6) and B16V^shUst(16) cells were analyzed for Itgb1 and Itgb3 expression by qRT-PCR. Expression was calculated as described in Materials & Methods (n = 6) and normalized to parental B16V cells. Itgb1 expression levels are significantly increased in B16V^shUst(16) cells compared to B16V^mock cells. Data are presented as the Fold-change±SEM.