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. 2004 Nov;186(22):7773–7782. doi: 10.1128/JB.186.22.7773-7782.2004

FIG. 5.

FIG. 5.

Identification and annotation of fine scale map differences. Genspect views show S. flexneri strain differences at selected map loci. Maps are represented as undulating bars, with restriction fragment sizes noted in kilobases. Pale blue arrows indicate deletions, deep blue arrows indicate insertions, and yellow arrows indicate differences between all strains. (A) Deletion in AMC[328Y] strain. Corresponding fragments in strains 301 and 2457T harbor glucosyltransferase genes. (B) Deletion in 301 strain. The indicated shrinking noted fragment, corresponding to the 2457T fragment, contains phage-related genes. (C) Extra restriction site in strains AMC[328Y] and 2457T caused by an insertion sequence element insertion. (D) 12.48-kb insertion in the 34.65-kb restriction fragment in 2457T. It contains iron uptake genes (sitA, -B, -C, and -D). (E) The 6.68-kb (strain 301) and 9.14-kb (strain 2457T) fragments contain the biosynthesis cofactors, the CobU, CobS, and CobT genes, and an alpha-helix protein gene. The two extra restriction fragments and additional sequence in 9.14-kb (strain 2457T) fragment contain several insertion sequence element insertions.