FIG. 3.

Analysis of RHA1 transformants containing origin of replication of pRHL3. Transformants were analyzed by PFGE (A) and Southern hybridization with a probe derived from the tsr gene (B). Lanes were loaded with the following: M, a chromosome size marker derived from Saccharomyces cerevisiae; 1, wild-type RHA1; and 2 to 4, independent transformants of RHA1 containing pCHB79. The positions of the RHA1 chromosome and each plasmid are indicated on the left. An arrow on the right indicates the deduced position of pCHB79, which corresponds to the origin of electrophoresis.