FIG. 4.

Replication origin region of pRHL3. (A) Subcloning of the origin of replication. Subclone plasmids are labeled, and their inserts are represented by horizontal thick bars. The results of transformation experiments are presented on the right: +, clones that yielded transformants of RHA1; −, clones that yielded no transformants. The six-digit numbers indicate the nucleotide positions of restriction sites on pRHL3. (B) Annotated ORFs in the replication origin region. ORFs are numbered according to Fig. 5 and are represented by horizontal arrows; rep1 is presented by a shaded arrow. A closed vertical arrowhead and an open box indicate the respective locations of the direct repeats and AT-rich region described in the text.