Effect of Lipitor and Celebrex on apoptosis of VCaP cells. VCaP cells were seeded at a density of 0.2×105 cells/ml and incubated for 24 h. The cells were then treated with Lipitor (10 μM) or Celebrex (10 μM) alone or in combination for 96 h. Apoptosis was determined by morphological assessment and caspase-3 assay. Each value is the mean ± S.E from three experiments. Differences for the number of apoptotic cells and caspase-3 activation between a combination group and a single agent-treated group were analyzed by ANOVA with the Tukey-Kramer multiple comparison test.