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. 2004 Dec;78(23):13163–13172. doi: 10.1128/JVI.78.23.13163-13172.2004

FIG. 5.

FIG. 5.

Antigen-specific IFN-γ SFCs isolated from the spleens of female and male BALB/c mice following repeated i.n. peptide immunizations. Groups of female and male BALB/c mice were immunized i.n. with 50 μg of peptide plus 1 μg of cholera toxin on days 0, 7, 14, and 28. On day 40, spleens were isolated and assayed by ELISpot for the frequency of P18-specific IFN-γ-secreting cells. Each bar is representative of the mean ± standard error of results from 12 mice. P values were 0.05 when P18-specific IFN-γ-secreting responses from females were compared to males.