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. 2004 Dec;78(23):12747–12761. doi: 10.1128/JVI.78.23.12747-12761.2004

FIG. 3.

FIG. 3.

Enhanced VSV replication in PERK−/− MEFs as a result of impaired eIF2α phosphorylation. PERK+/+ and PERK−/− MEFs were infected (lanes 2 to 4 and lanes 6 to 8) or not infected (lanes 1 and 5) with VSV at MOIs of 1 (A), 10 (B), or 50 (C). Protein extracts were harvested at the indicated times postinfection and subjected to immunoblot analysis by using the rabbit polyclonal anti-phosphoserine 51 eIF2α antibody (top panels) or with the eIF2α panspecific antibody (lower panels). The ratio of phosphorylated to total eIF2α protein for each lane is indicated. (D) PERK+/+ and PERK−/− MEFs were treated with 1 μM TG for 2 h (bottom panels), infected (middle panels) or not infected (top panels) with VSV at an MOI of 10, and harvested at 12 hpi. Protein extracts (80 μg) were subjected to 2D electrophoresis and immunoblot analysis with a rabbit polyclonal anti-phosphoserine 51 eIF2α antibody.