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. 2004 Dec;78(23):12747–12761. doi: 10.1128/JVI.78.23.12747-12761.2004

FIG. 5.

FIG. 5.

VSV-induced apoptosis of PERK−/− MEFs proceeds through caspase-12 activation. (A) Protein extracts from PERK+/+ and PERK−/− MEFs infected with VSV (MOI = 1) were collected at different times postinfection and subjected to immunoblot analysis with a rabbit polyclonal antibody to caspase-12. The upper band represents the inactive protease, whereas the lower band represents the cleaved and active enzyme. (B and C) PERK+/+ and PERK−/− MEFs were either left untreated or treated with caspase inhibitors (zVAD-fmk; 10 μM) 2 h before infection with VSV (MOI = 2) for 24 h. Cells were photographed at ×100 magnification (B), or protein extracts (25 μg) were subjected to immunoblot analysis with a rabbit polyclonal antibody to PARP (C). The upper band represents the full-length 116-kDa PARP protein, and the lower band represents the cleaved 89-kDa PARP.