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. 2004 Dec;78(23):12829–12837. doi: 10.1128/JVI.78.23.12829-12837.2004

FIG. 1.

FIG. 1.

Enrichment of hairpin ribozymes that inhibit HIV infection. (A) Flow cytometry analysis of GFP expression in HIV-1LAI-infected CEM-GFP cells expressing Rz-Lib. CEM-GFP cells in the absence of HIV-1LAI (top left) and CEM-GFP minus Rz-Lib plus HIV-1LAI (top right) are controls. The 1st Rz-Lib is the original ribozyme library, while the 2nd to 4th Rz-Libs represent the mini-libraries generated in subsequent rounds of selection (see Materials and Methods for the construction of mini-libraries). The percentages of GFP-negative cells in each round of selection are shown. (B) Two-color fluorescence-activated cell sorter analysis of intracellular p24 (Cy-5) and GFP expression in HIV-1LAI-infected cells transfected with the 4th Rz-Lib (middle panel) or no ribozyme (right panel). Decrease in GFP+ Cy-5+ cells indicates silencing of HIV-1 gene expression and inhibition of HIV-1 replication.