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. 2004 Dec;78(23):12829–12837. doi: 10.1128/JVI.78.23.12829-12837.2004

FIG. 2.

FIG. 2.

FIG. 2.

Confirmation of individual protective ribozymes. (A) Protection of CEM-GFP from HIV infection by IVRz-1. CEM-GFP cells expressing IVRz-1 were infected by HIV for 6 days and then analyzed for GFP expression. (B) IVRz-1 inhibits HIV spread in CEM-GFP cells. Culture media of the infected cells in panel A were collected at various time points postinfection and assayed for p24. A 2 μM concentration of zidovudine was included as a positive control for viral inhibition. (C) MAGI cells transfected with 17 individual ribozymes and a control ribozyme were infected with HIV. The percentage of infected (blue) cells relative to the control ribozymes is shown. (D) The same cells as shown in panel A were infected with VSV-G-pseudotyped HIV and similarly analyzed. The infection with either the wild-type or the pseudotyped virus was performed at least three times, and the result of a representative experiment is shown.