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. 2004 Dec;78(23):13315–13324. doi: 10.1128/JVI.78.23.13315-13324.2004

FIG. 4.

FIG. 4.

Enrichment of aberrant ends during and after integration and restriction endonuclease cleavage assay to screen for consensus 2-LTR circle junctions. (A) Integration of correct linear viral DNAs enriches for linear DNAs with aberrant ends, which can then be ligated to form 2-LTR circles. The vertical bar on the left represents the total population of linear viral DNAs. Integration-competent ends are represented by the shaded portions of the bar, and integration-defective ends are represented by the white portion. Integration is efficient (see Discussion), and the majority of linear viral DNAs containing correct ends become integrated to form proviruses. The consensus 2-LTR circle junctions that contain complete ends have a ScaI recognition sequence at the 2-LTR circle junction. (B) Representative picture of a gel in which ScaI endonuclease cleavage was used to screen for consensus 2-LTR circle junctions. Lane 1 contains the 1-kb ladder (Invitrogen), lane 2 contains an aberrant 2-LTR circle junction, and lane 3 contains a consensus 2-LTR circle junction.