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Schematic of the TSWV glycoprotein ORF and of soluble, truncated GN (GN-S). The top figure represents the precursor polyprotein, with putative signal sequences, signal peptidase cleavages sites, N- and O-linked glycosylation sites, and transmembrane domains indicated. The bottom figure is a schematic of GN-S, from amino acids 35 to 309, expressed from a baculovirus. Note that the putative hydrophobic domains were removed and six-His tags were added. The figure is not drawn to scale.
