FIG. 5.

Analysis of soluble G_N (G_N-S) glycosylation. Purified G_N-S was incubated with glycosidases to remove oligosaccharides and then separated by SDS-PAGE. The proteins were transferred to nitrocellulose membranes, and G_N-S was detected with a six-His MAb. (A) G_N-S incubated with enzymes to remove oligosaccharides. Lane 1, O-linked glycans; lane 2, N-linked glycans; lane 3, mock digestion, no glycosidases; and lane 4, N- and O-linked glycans. (B) Short exposure of panel A showing the differences in size of G_N-S incubated with enzymes to remove N-linked glycans (lane 2) and with no enzymes for a mock digestion (lane 3).