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. 2004 Dec;78(23):13197–13206. doi: 10.1128/JVI.78.23.13197-13206.2004

FIG. 6.

FIG. 6.

Analysis of GN and GN-S dimerization. Increasing amounts of β-ME were added to TSWV purified from infected plants (GN) or to GN-S. Proteins were detected by Western blotting. (A) Purified TSWV detected with GN MAb. Lane 1, no β-ME and sample was not boiled; lane 2, no β-ME; lane 3, 0.1% β-ME; lane 4, 1.0% β-ME; lane 5, 2.5% β-ME; and lane 6, 5% β-ME. (B) Purified GN-S protein was detected with a six-His MAb. Lane 1, no β-ME and sample was not boiled; lane 2, no β-ME; lane 3, 0.1% β-ME; lane 4, 1.0% β-ME; lane 5, 2.5% β-ME; and lane 6, six-His-tagged molecular weight marker.